2001 Ccm1, a regulatory gene controlling the induction of a carbon-concentrating mechanism in Chlamydomonas reinhardtii by sensing CO 2 availability. thesis, West Virginia University, USA.įukuzawa H., Miura K., Ishizaki K., Kucho K.
2012 Growth of Chlorella vulgaris and Chlamydomonas reinhardtii for biodiesel production and carbon dioxide capture. 2012 Transcriptome-wide changes in Chlamydomonas reinhardtii gene expression regulated by carbon dioxide and the CO 2-concentrating mechanism regulator CIA5/CCM1. 16, 12–19.įang W., Si Y., Douglass S., Casero D., Merchant S. 2016 Transcriptome-based global analysis of gene expression in response to carbon dioxide deprivation in the green algae Chlorella pyrenoidosa. 129, 439–445.Įarth System Research Laboratory 2020 Trends in atmospheric carbon dioxide ( ).įan J., Xu H.
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and Scenedesmus obliquus cultivated in a three-stage serial tubular photobioreactor. 2007 Biofixation of carbon dioxide by Spirulina sp. 2020 A system dynamics simulation applied to healthcare: a systematic review. 2021 Modeling of CO 2 capture ability of ionic liquid using connectionist smart paradigms. 2017 A novel activation domain is essential for CIA5-mediated gene regulation in response to CO 2 changes in Chlamydomonas reinhardtii.
thesis, Iowa State University, USA.Ĭhen B., Lee K., Plucinak T., Duanmu D., Wang Y., Horken K. 2016 The function and regulation of CIA5/CCM1 in Chlamydomonas reinhardtii. 2011 RNA-mediated silencing in algae: biological roles and tools for analysis of gene function. 2014 CO 2 biofixation and carbonic anhydrase activity in Scenedesmus obliquus SA1 cultivated in large scale open system.
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2014 CRISPR-Cas systems: prokaryotes upgrade to adaptive immunity. 2021 Optimization of Chlamydomonas reinhardtii cultivation with simultaneous CO 2 sequestration and biofuels production in a biorefinery framework. 2018 Improvements in algal lipid production: a systems biology and gene editing approach. 6, 1–7.īanerjee A., Banerjee C., Negi S., Chang J.
2016 DNA-free two-gene knockout in Chlamydomonas reinhardtii via CRISPR-Cas9 ribonucleoproteins. Nevertheless, for mutant species in the same concentrations, the observed removal efficiencies were 16%, 23% and 9%.īaek K., Kim D.
Additionally, our findings revealed that the control type isolate in CO 2 concentrations of 0.04%, 0.5% and 1% had removal efficiencies of 27%, 37% and 21%, respectively. However, the difference between the control microalgae species and the mutant species was in the CO 2 removal efficiency. reinhardtii has better performance (i.e., response to CO 2 treatment) in both control and mutant species at 0.5% CO 2 concentration than other concentrations. reinhardtii to perform CO 2 sequestration. In this research, we knocked out the Cia5 gene using the CRISPR/Cas9 technique and analysed the ability of C. Among several genes in algae that respond to CO 2 and regulators control the expression of each Cia5 is one of the most critical transcriptional regulators. Besides, this technology can play a role in the fight against greenhouse gases (e.g., carbon dioxide) production by studying genetic pathways to improve algal strains. CRISPR/Cas9 technology is one of the common methods of genome editing and targeted gene mutation, which has recently been used for manipulating microalgae such as Chlamydomonas reinhardtii.
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